First, you must determine the length of your menstrual cycle. This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding begins again. Please refer to the chart below to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform one test each day over a five-day period or until the LH surge has been detected. Please refer to the table to the left for information on when to start using ovulation tests.
|Your Cycle Length||Start To Test|
|21 days||Day 6|
|22 days||Day 6|
|23 days||Day 7|
|24 days||Day 7|
|25 days||Day 8|
|26 days||Day 9|
|27 days||Day 10|
|28 days||Day 11|
|29 days||Day 12|
|30 days||Day 13|
|31 days||Day 14|
|32 days||Day 15|
|33 days||Day 16|
|34 days||Day 17|
|35 days||Day 18|
|36 days||Day 19|
|37 days||Day 20|
|38 days||Day 21|
STEP 1: Bring the test pouch to room temperature (18-30°C). Collect urine into a clean container (such as plastic cup). The best way to collect sample is by placing a cup in the middle of the urine stream. To begin testing, open the sealed pouch by tearing along the notch. Remove the test from the pouch when you are ready to use it.
STEP 2: Immerse the testing strip into the urine with the arrow end pointing towards the urine. Do not immerse past the MAX (maximum) line. Take the testing strip out after 5 seconds and lay it flat on a clean, dry, non-absorbent surface (e.g., mouth of the urine container). Do not immerse for longer than 7 seconds. Note the time.
STEP 3: Wait for colored bands to appear. Depending on the concentration of LH in the test specimen, positive results may be observed in as short as 40 seconds. However, to confirm negative results, the complete reaction time (10 minutes) is required. Do not read the results after 30 minutes as this type of test is designed for rapid determination only.
As the LH One Step Ovulation Test begins to work, a colored band will appear in the upper section of the result area to show that the test is working properly. This band is the Control Band (C). The lower section of the result area indicates the test results. If another colored band, the Test Band (T), appears at the lower section of the result area and is of equal or darker color than the Control Band, the results should be interpreted as a positive test.
Only one color band appears on the control region or if the test line is present but fainter than the control line the result is negative.
If 2 color bands are visible and the test band is equal to or darker than the control band, this means that you will probably ovulate in the next 24-48 hours.The best time for egg fertilization is between 24-48 hours
No visible bands in the control and test regions. Make sure to follow the above specified instructions for optimum results. This result is extremely unlikely. If this happens, you should repeat the test by using a new LH One-Step Ovulation Test. Test results may vary for different individuals depending on the concentration of LH your body produces, as well as monitoring frequency, and testing technique. The best method is to test once a day, preferably later in the afternoon or early evening since LH is best synthesized during active period.
When to Stop Testing:
Unless otherwise specified by your doctor, stop testing once the LH surge is detected.
Note: If you suspect that you have ovulation problems, or problems with detecting LH surge, please see your physician. There are numerous stimulatory procedures that can induce the ovulation surge. This type of rapid test is used for preliminary qualitative screening of ovulation surge. If you need any assistance please contact your health care provider.